ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of Rho-GDI in the decidual tissues of patients preeclampsia and explore its clinical implication.</p><p><b>METHODS</b>Real-time PCR, Western blotting and immunohistochemistry were used to detect the mRNA and protein expressions of Rho-GDI in the decidual tissues from 30 normal women with full-term pregnancy, 30 patients with early-onset severe preeclampsia and 30 with late-onset severe preeclampsia.</p><p><b>RESULTS</b>Rho-GDI expression was found mainly on the cell membrane and in the cytoplasm and nuclei of the decidual cells, occasionally occurring in the stroma. Both the mRNA and protein expressions of Rho-GDI in the decidual tissues were significantly higher in the normal pregnancy group than in the two severe preeclampsia groups (P<0.05), and the patients with late-onset severe preeclampsia had the lowest expressions of Rho-GDI.</p><p><b>CONCLUSION</b>The lowered expression of Rho-GDI in the deciduas might be involved in the pathogenesis and progression of preeclampsia.</p>
Subject(s)
Adult , Female , Humans , Pregnancy , Young Adult , Decidua , Metabolism , Guanine Nucleotide Dissociation Inhibitors , Genetics , Metabolism , Pre-Eclampsia , Metabolism , RNA, Messenger , Genetics , Metabolism , Real-Time Polymerase Chain Reaction , Methods , rho-Specific Guanine Nucleotide Dissociation InhibitorsABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of annexin V in the decidua tissues of preeclampsia patients and explore its clinical significance.</p><p><b>METHODS</b>Real-time PCR, Western blotting and immunohistochemistry were employed to detect the mRNA and protein expressions of annexin V in the deciduas from 35 normal pregnant women at full term, 38 early onset severe preeclampsia patients and 33 late onset severe preeclampsia patients.</p><p><b>RESULTS</b>Annexin V was found on the cell membrane and in the cytoplasm of the decidual cells and stroma. Both the mRNA and protein of annexin V expressions in the decidua tissues were significantly different between normal pregnancy group and early or late onset severe preeclampsia group (P<0.05), being the highest in normal pregnancy group and the lowest in early onset severe preeclampsia group.</p><p><b>CONCLUSION</b>The low expression of annexin V in the deciduas might participate in the hypercoagulability state in preeclampsia patients.</p>